Imaging with Raman photons: a novel use of mixed-mode spectroscopy

Abstract Surface enhanced Raman spectroscopy is today an established technique used for chemical fingerprinting. Here, we showcase engineered hierarchical substrate, in which the plasmonically active regions, restricted to a micron scale, two dimensional hexagonal pattern are examined. Spatial variation of signal from any analyte, uniformly coating consequently bears high registry with underlying pattern. This spatially contrasted enhancement allows optical imaging 2D solely using scattered photons analyte. While brightness and contrast determine analyte identification detection sensitivity, hyperspectral can be exploited increasing specificity. Proof concept demonstration carried out via acquisition images rhodamine fluorescein dyes then applied detect glucose 40 mM concentration. The large area requirement long-range uniformity detected patterns positive detection, implemented machine learning based recognition protocol also increases statistical confidence detection. simultaneous, sacrifices continuous spectral information at cost speed, reproducibility minimising human error automation presented here.